Sinapine Thiocyanate Ameliorates CCl4-Induced Hepatic Fibrosis by Activating Nrf2 and Suppressing NF-kappaB/TGF-beta1 Signaling.

Hepatic fibrosis, characterized by excessive extracellular matrix deposition, lacks effective pharmacotherapy. This study investigates the hepatoprotective effects of Sinapine Thiocyanate (STC), a phenolic alkaloid from Brassica rapa, against CCl4-induced liver fibrosis in mice. Using a combination of hepatic transcriptomics (RNA-seq), serum metabolomics (LC-MS), and gut microbiota profiling (16S rRNA sequencing), we elucidate the molecular mechanisms involving Nrf2/NF-kappaB/TGF-beta1 signaling and gut-liver axis modulation. Our findings demonstrate that STC dose-dependently attenuates liver fibrosis and restores redox homeostasis, positioning it as a promising natural anti-fibrotic therapeutic candidate