Supplementary Data: Effects of the Periparturient Period and Parity on Local Innervation Markers in Subcutaneous Adipose Tissue of Dairy Cows (Sparks et al., 2026)

This supplementary data is for the article: "Effects of the Periparturient Period and Parity on Local Innervation Markers in Subcutaneous Adipose Tissue of Dairy Cows" in the Journal of Dairy Science.

Supplementary Table 1. Multivariable modeling of period, parity, and period*Lactation using subcutaneous adipose tissue collected from 16 multiparous dairy cows (3.5 ± 1.6 lactations, n=16) from the prepartum period (8.56 ± 2.3 days to parturition, mean ± SD, n=16) and the postpartum period (+7 days after parturition, n=16).

Supplementary Figure 1 Changes in the subcutaneous adipose tissue of prepartum (8.563 ± 2.83 days to parturition, mean ± SD, n=16) and postpartum (+7 days after parturition, n=16) multiparous dairy cows (3.5 ± 1.6 lactations, n=16). A. Representative image of total protein staining using Pierce™ Reversible Protein Kit. B. Beef thalamus, spinal cord, and cortex isolated for positive control of calcitonin gene-related (CGRP) product shown as proCGRP. C-E. Gene expression of MPZ, S100B, NGFR normalized to pan-Schwann cell marker SOX10. F. Visualization of Spearman correlation between postpartum S100B with postpartum EBF2, TMEM26, and PRDM16 protein abundance. Protein abundance and gene expression analyzed by a repeated measures mixed-effects model with the repetition of period by cow nested in parity, and parity, period, and the interaction of parity and period as fixed effects. *p≤0.05