Computational code underlying the publication “Engineered patterns of Notch ligands Jag1 and Dll4 elicit differential spatial control of endothelial sprouting”

Spatial regulation of angiogenesis is important for the generation of functional engineered vasculature and the Notch ligands Jag1 and Dll4 are known to regulate endothelial sprouting. In this project, <em>in vitro </em>and <em>in silico </em>models were used to analyze the ability of micropatterned Jag1 and Dll4 to spatially control the sprouting process. Dll4 patterns, but not Jag1 patterns, were found to elicit spatial control and computational simulations suggest different timing of Notch activation by Jag1 and Dll4 to be the reason for their distinct effects. The more potent spatial control of sprouting by Dll4 in comparison to Jag1 could be exploited as a tool in vascular engineering and regenerative medicine. <br> This dataset contains computational modeling data simulating cell-cell signaling mediated by Notch ligands Jag1 and Dll4 during controlled endothelial sprouting.

血管生成的空间调控对于功能性工程化血管的构建至关重要，而Notch配体（Notch ligands）Jag1与Dll4已被证实可调控内皮细胞出芽过程。本研究采用体外（in vitro）与计算机模拟（in silico）模型，分析微图案化Jag1与Dll4对出芽过程的空间调控能力。研究发现，仅Dll4图案可引发有效的空间调控，计算机模拟结果显示，Jag1与Dll4对Notch激活的时序差异正是二者调控效果迥异的核心原因。相较于Jag1，Dll4对内皮出芽过程具备更强的空间调控能力，这一特性可作为工具应用于血管工程学与再生医学领域。
本数据集包含受控内皮细胞出芽过程中，由Notch配体Jag1与Dll4介导的细胞间信号传导的计算机模拟建模数据。