Reconstitution of Human Brain Cell Diversity in Organoids via Four Protocols [scRNA-seq]

Systematic analysis of the cellular and transcriptional landscape of brain organoids grown from multiple cell lines using four different protocols recapitulating dorsal and ventral forebrain, midbrain, and striatum via single-cell RNA sequencing. Single-cell RNA-sequencing of brain organoids at day 120 grown in four distinct growth protocols designed to generate dorsal (D) and ventral (V) forebrain, midbrain (M) and striatum (S) tissue using eight different pluripotent stem cell lines. Per cell line-protocol combination two organoids (biological replicates) were grown. For cell line 176 the experiment was conducted in two independent biological replicates (repetitions E1 and E2). *************************************************************** Submitter states that missing raw data are being made available for controlled access in EGA. *************************************************************** Raw data have been deposited at the European Genome-phenome Archive (EGA), under series numbers EGAS50000000662, and are available through controlled access.