In vitro plaque formation model to unravel biofilm formation dynamics on implant abutment surfaces

Biofilm formation on implant-abutment surfaces can cause inflammatory reactions. Ethical concerns often limit intraoral testing, necessitating preliminary in vitro or animal studies. Here, we propose an in vitro model using human saliva and hypothesize that this model has the potential to closely mimic the dynamics of biofilm formation on implant-abutment material surfaces in vivo. 16S-rRNA was one of analyses used in the study to assess relative abundance of oral bacteria from saliva inoculum, in vivo biofilm, and in vitro biofilm.