Transcriptome analysis of entorhinal cortex tissue from neuron-specific Sec61a1-overexpressing transgenic mice after AAV-mediated knockdown of Sec61a1 or Adarb2

Expression profiling by high throughput sequencingSEC61A1 is essential for protein translocation and ER function, while ADARB2 is an RNA editor that binds to dsRNA without degradation capability. Previous studies suggest that SEC61A1 overexpression may lead to mitochondrial dsRNA accumulation. To investigate this, we utilized adeno-associated virus (AAV)-mediated shRNA knockdown of Sec61a1 or Adarb2 in the cortex of neuron-specific SEC61A1-overexpressing transgenic mice. After two months, bulk RNA sequencing was performed on cortical tissue to examine transcriptomic changes. This study aims to explore how SEC61A1 overexpression contributes to mitochondrial dsRNA accumulation and how ADARB2, as a dsRNA-binding protein, may influence related pathways.