Transcriptomic profiling of human alveolar organoids(hALOs)

We performed global transcriptomic profiling of hALOs at early (P4), middle (P13) and late (P20) passages and found that the transcripts of the hALOs were similar to that of primary lung progenitor organoids.hALOs highly expressed genes associated with lung development/lung progenitor, AT2 specification and AT1 specification.We found that our hALOs were more similar to pseudoglandular-staged lung.The gene sets associated with alveolar maturation, such as surfactant homeostasis or response to oxygen levels, were upregulated in P20. Besides, genes involved in endoderm development or respiratory tube development were downregulated Overall design: To determine how similar the transcriptomic identities of hALOs were to primary human lung, we performed RNA-sequencing (RNA-seq) of hALOs at early (P4), middle (P13) and late (P20) passages and compared our data with lung adenocarcinomas cancer cell line A549, H9 hESCs, human fetal lung and primary lung progenitor organoids from publicly available databases