Micrographs (A) and quantification of total colony forming units-fibroblasts (CFU-F; B), unmineralized ALP colonies (CFU-ALP; C) and mineralized ALP colonies (CFU-O; D) in bone marrow stromal cell cultures of female mutant () and WT () mice

Values are the mean ± the SEM of 8–15 wells. (E) Micrographs of and female spleen cells grown with RANKL+M-CSF and stained for TRAP activity (TRAP); (F) number of TRAP cells; (G) number of osteoclasts (TRAP cells with ≥3 nuclei) formed in spleen cell and bone marrow cultures. Values are the mean ± the SEM of 12 wells. (H) Number of resorption pits on dentine slices plated with and differentiated osteoclasts; values are the mean ± the SEM of six slices. ***, P &lt; 0.001; ****, P &lt; 0.0001 versus matched WT.<b>Copyright information:</b>Taken from "Bone sialoprotein plays a functional role in bone formation and osteoclastogenesis"The Journal of Experimental Medicine 2008;205(5):1145-1153.Published online 12 May 2008PMCID:PMC2373846.