Data underlying the publication: Discrete long-range on-cell motion of bacteriophage T4

Interaction dynamics between phage T4 and host E. coli B revealed during single-particle tracking using fluorescence microscopy in HILO mode. Image acquisition of T4 phage trajectories was performed at a frame rate of 50 (files with date: 2018-03-13) or 200 Hz (files with date: 2018-12-04) with a pixel resolution of 80 or 161 nm, respectively. Phage trajectories were extracted from the sequences of images using the algorithm TrackMate in ImageJ (.XML files). Data can be imported in matlab (.mat file). <em>E. coli</em> B bacteria were immobilized at the surface of the flow cell and visualized using phage contrast microscopy. Data of phage trajectories can be overlaid with corresponding images of bacteria to study the initial phage-bacteria interaction dynamics at single-particle level. Data obtained at 2018-03-13 consist out of three individual experiments (flow cell chambers), each containing several movies of different fields of view. Data obtained at 2018-12-04 consist out of one experiment (flow cell chamber) during which several movies were collected at different fields of view. Detailed information can be found in material and methods section of the paper.

本数据集展示了通过HILO模式荧光显微镜开展单粒子追踪实验时，所揭示的T4噬菌体与宿主大肠杆菌（E. coli）B菌株之间的相互作用动力学特征。T4噬菌体运动轨迹的图像采集帧率分别为50 Hz（对应2018年3月13日的数据集文件）与200 Hz（对应2018年12月4日的数据集文件），对应的像素分辨率分别为80 nm与161 nm。研究人员采用ImageJ软件中的TrackMate算法从图像序列中提取噬菌体运动轨迹，提取结果保存为XML格式文件；该数据集可导入至MATLAB中进行分析，导入格式为MAT文件。实验中将大肠杆菌（E. coli）B菌株固定于流动池表面，并通过噬菌体衬度显微镜完成成像观测。可将噬菌体运动轨迹数据与对应细菌成像图进行叠加，以单粒子水平解析噬菌体与细菌的初始相互作用动力学过程。2018年3月13日采集的数据集包含3组独立实验（对应3个流动池室），每组实验均包含多个不同视场的成像影片。2018年12月4日采集的数据集仅包含1组实验（对应1个流动池室），实验期间采集了多个不同视场的成像影片。数据集的详细信息可参阅论文的材料与方法部分。