Average amount of gold particles on the membrane of LDs in cells that had been transfected with Jc1 wild type or Jc1/ΔE1E2 or Jc1/Δ2328-2435 (exemplified by Figure 7B).

The data are based on two different labeling experiments, two grids per experiment considering 20 lipid droplets per grid. Only the labeling on the membrane around the LD was considered, and not labeling on adjacent vesicles or the ER membrane. Statistically significant differences were only observed in case of core labeling between Jc1 wild type and the two mutants (p-value <0.0001 as determined by t-test), but not between the two mutants. Likewise, labeling density of NS5A did not differ between the three constructs in a statistically significant manner.