Effect of formoterol and tumor necrosis factor (TNF) alpha alone and in combination on gene expression changes in human primary bronchial epithelial cells: a comparison with forskolin treatment. Homo sapiens

RNA sequencing was performed to study the genomic effects of formoterol, a long acting beta-2-adrenoceptor agonist, alone and in combination with the pro-inflammatory cytokine, TNF alpha in human primary bronchial epithelial cells, and to compare its effect to forskolin. Our results show that formoterol promoted a profound genomic response alone and on background of an inflammatory stimulus. Moreover, formoterol and forskolin regulated transcriptomes were highly rank order correlated which suggests that formoterol-mediated gene expression changes are cAMP dependent