Cardinium and Wolbachia co-infection of haplodiploid Pezothrips kellyanus induces cytoplasmic incompatibility, male development and embryonic mortality and post-embryonic mortality

Data describes multiple crossing experiments involving Pezothrips kellyanus laboratory colonies that are infected with with Cardinium (Ic), infected with both Cardinium and Wolbachia (Icw), or uninfected by either endosymbiotic bacteria (U). Pezothrips exhibit haplodiploid reproduction and the fecundity and reproductive effects of the endosymbiont complement in various mating combinations, as well as in virgin females, was measured to determine the reproductive outcomes. This data demonstrates the stability of co-infection, the high titre of Wolbachia infection relative to Cardinium in each co-infected individual, and the apparent lack of competition between these bacteria within hosts. Maternal transmission to offspring was complete for Wolbachia and high for Cardinium. Cardinium-induced CI resulted in a combination of male development (MD) and embryonic female mortality of fertilised eggs, and Wolbachia-induced CI resulted in female mortality together with post-embryonic mortality.

本数据集涵盖多项针对凯利蓟马（Pezothrips kellyanus）实验室种群的杂交实验，实验种群分为三类：仅感染卡氏菌属共生菌（Cardinium，记为Ic）、同时感染卡氏菌属与沃尔巴克氏体属共生菌（Wolbachia，记为Icw），以及未被任意一种内共生菌感染的空白对照组（记为U）。蓟马类昆虫具有单倍二倍体生殖模式，本实验通过测定不同交配组合下以及处女雌虫体内共生菌组合对繁殖力与生殖表现的影响，以明确其生殖结局。本数据集揭示了共感染的稳定性、共感染个体中沃尔巴克氏体滴度显著高于卡氏菌属的现象，以及两种共生菌在宿主体内几乎不存在竞争关系的特征。沃尔巴克氏体可完全经母系传递至子代，卡氏菌属的母系传递效率亦处于较高水平。卡氏菌属诱导的胞质不亲和（Cytoplasmic Incompatibility, CI）会导致受精卵出现雄性发育（Male Development, MD）与雌性胚胎死亡的混合表型；而沃尔巴克氏体诱导的胞质不亲和则会同时引发雌性死亡与胚胎后期死亡。