Methylobacterium strains isolated from the phyllosphere of temperate forests in the province of Quebec, Canada.. Methylobacterium sp.

Methylobacterium strains (Bacteria, Proteobacteria, Alphaproteobacteria, Rhizobiales, Methylobacteriaceae) isolated from leaf samples from two forests in the province of Quebec, Canada: Mont Saint Hilaire (MSH) and Station Biologique des Laurentides (SBL). Strains were isolated on minimum salt media with methanol 0.1% as sole carbon source. Each tree was sampled either once in August 2017 in MSH (19 trees, pilot survey), or 3-4 times from June to October 2018 in both MSH and SBL (52 trees, seasonal survey). (i) 80 strains and identified by Sanger-sequencing of the 16S V4 region. (ii) 20 strains isolated from the pilot survey used to develop a Methylobacterium-specific marker by Sanger-sequencing of three and two hypervariable regions of genes sucA and rpoB, respectively. (iii) 167 strains isolated from the seasonal survey and identified by Sanger sequencing of rpoB.