Data for paper "A new mode of contrast in biological second harmonic generation microscopy"

Enhanced image contrast in biological
second harmonic imaging microscopy (SHIM) has previously been reported via
quantitative assessments of forward- to epi-generated signal intensity ratio
and by polarization analysis. Here we demonstrate a new form of contrast: the
material-specific, wavelength-dependence of epi-generated second harmonic
generation (SHG) excitation efficiency, and discriminate collagen and myosin by
ratiometric epi-generated SHG images at 920 nm and 860 nm.

Collagen shows increased SHG
intensity at 920 nm, while little difference is detected between the two for
myosin; allowing SHIM to characterize different SHG-generating components
within a complex biological sample.

Scientific Reports 7(1) Article number:13331 (2017)

DOI: 10.1038/s41598-017-13752-y