RNA pull-down analysis of hsa_circ_0001400 in 293T cells
收藏NIAID Data Ecosystem2026-05-02 收录
下载链接：
https://www.ncbi.nlm.nih.gov/sra/SRP383560
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资源简介：
293T cells are transfected with hsa_circ_0001400 expression plasmid vectors for 24 hours. Cells are then incuvated with Psoralen, exposed to long-wave UV for 10 minutes, and total RNA was extracted. Biotynilated DNA oligos sense (control) or antisense of circ_0001400 are added and circRNA-RNA complexes are enriched using streptavidin beads. RNA was cleaned and exposed to short-wave UV and subjected for RNA-Seq. Overall design: Triplicates of 293T cells are pulled-down for circ_0001400 using antisense DNA probes or control.
创建时间：
2025-06-14
