Geographic coordinates and allele scores of Puccinia psidii samples from Brazil and Uruguay

Data include geographic coordinates of single uredinial pustules of Puccinia psidii ca. 6 mm diameter collected between March 2008 and August 2009 from 148 individual plants representing seven myrtaceous taxa (eucalypts, guava, rose apple, Brazilian guava, Java plum, jabuticaba, and pitanga) in nine Brazilian states and one location in Uruguay. Samples were genotyped at 10 microsatellite loci (PpSSR012, PpSSR014, PpSSR018, PpSSR022, PpSSR087, PpSSR102, PpSSR146, PpSSR161, PpSSR178, and PpSSR195) using genomic DNA extracted directly from each pustule following a modified CTAB-based protocol. Fragment analysis was via capillary electrophoresis using an ABI 3700 DNA automated sequencer. Positive and negative controls were included for each locus scored and scoring was repeated for representative alleles for each locus to ensure the accuracy of genotyping. Allele sizes were estimated using marker standards (ROX Geneflo 625, CHIMERx, Milwaukee, WI, USA) and scored using ABI PeakScanner Analysis Software v1.0.