Geographic coordinates and allele scores of Puccinia psidii samples from Brazil and Uruguay

Data include geographic coordinates of single uredinial pustules of Puccinia psidii ca. 6 mm diameter collected between March 2008 and August 2009 from 148 individual plants representing seven myrtaceous taxa (eucalypts, guava, rose apple, Brazilian guava, Java plum, jabuticaba, and pitanga) in nine Brazilian states and one location in Uruguay. Samples were genotyped at 10 microsatellite loci (PpSSR012, PpSSR014, PpSSR018, PpSSR022, PpSSR087, PpSSR102, PpSSR146, PpSSR161, PpSSR178, and PpSSR195) using genomic DNA extracted directly from each pustule following a modified CTAB-based protocol. Fragment analysis was via capillary electrophoresis using an ABI 3700 DNA automated sequencer. Positive and negative controls were included for each locus scored and scoring was repeated for representative alleles for each locus to ensure the accuracy of genotyping. Allele sizes were estimated using marker standards (ROX Geneflo 625, CHIMERx, Milwaukee, WI, USA) and scored using ABI PeakScanner Analysis Software v1.0.本数据集涵盖2008年3月至2009年8月间采集的148株单株寄主的桃金娘柄锈菌（Puccinia psidii）单夏孢子堆疱斑（直径约6mm）的地理坐标，这些寄主隶属于7个桃金娘科类群（桉树、番石榴、蒲桃、巴西番石榴、爪哇蒲桃、嘉宝果与毕当茄），分布于巴西9个州及乌拉圭1处采样点。所有样本均针对10个微卫星位点（microsatellite loci）进行基因分型，所用位点为PpSSR012、PpSSR014、PpSSR018、PpSSR022、PpSSR087、PpSSR102、PpSSR146、PpSSR161、PpSSR178及PpSSR195；基因组DNA直接从每个疱斑中提取，采用改良的十六烷基三甲基溴化铵（CTAB）提取法。片段分析采用毛细管电泳，使用ABI 3700型全自动DNA测序仪完成。每个参与分型的位点均设置阳性与阴性对照，且对每个位点的代表性等位基因进行重复分型，以确保基因分型结果的准确性。等位基因片段大小通过分子量标准物ROX Geneflo 625（CHIMERx，美国威斯康星州密尔沃基市）进行估算，分型工作采用ABI PeakScanner分析软件v1.0完成。