Filtrate from UBA acid mine drainage sample. mine drainage metagenome

Small size selected cells (<450 nm) by filtration of Acid mine drainage from the UBA site at Richmond Mine at Iron Mountain, California. This library was constructed by filtering the Ultraback A drift Iron Mountain biofilm through a 450 nm filter to enrich for ultra-small Archaea, called ARMAN. 60 Mbs of that filtrate was then Sanger sequenced and near complete genomes of two of the ARMAN groups were obtained with coverage up to 15X. The two genomes have been named Candidatus Parvarchaeum acidiphilum (ARMAN-4) and Candidatus Parvarchaeum acidophilus (ARMAN-5).