Screening the Homologous Food-Medicine Kelp as a Dietary Intervention for Fructose-accelerated colitis-associated colorectal cancer tumorigenesis

To further elucidate the mechanisms by which high-fructose intervention promotes colorectal cancer, we conducted single-cell RNA sequencing (scRNA-seq) on colon tissue samples collected from mice subjected to various treatments. Specifically, we obtained colon tissues from mice challenged with water (Nor), AOM/DSS at 30 and 75 days (A1, A2) and AOM/DSS combined with fructose supplementation at 30 and 75 days (F1, F2). This comprehensive approach allowed us to investigate the transcriptional changes at the single-cell level and gain deeper insights into the role of fructose in CRC development.