Dataset of microfilaremia and duplex qPCR (Dirofilaria immitis and Wolbachia sp.) molecular screening in a comparative study of oral treatments with milbemycin oxime and doxycycline in client-owned dogs naturally infected with D. immitis

This dataset contains the longitudinal monitoring data from a 12-month comparative study evaluating oral treatments with milbemycin oxime (MBO) and a combination of milbemycin oxime + doxycycline (MBO+Doxy) in client-owned dogs naturally infected with Dirofilaria immitis. The data provided represents the quantitative results of parasitological and molecular screenings conducted throughout the study period. The parameters included in this dataset comprise measurements of microfilaremia, quantified as the number of microfilariae per mL of blood assessed via the modified Knott’s technique, and molecular screening results derived from a duplex qPCR assay. Molecular data are presented as Cycle Threshold (CT) values, which were obtained to simultaneously detect and quantify Dirofilaria immitis and D. immitis-specific Wolbachia sp. DNA. Included columns provide the ID as a unique identifier for each dog, the designated Treatment Group, the monitoring Day expressed in days post-treatment (dpt), the microfilariae count (mff), and the Cycle Threshold values for Dirofilaria immitis (Diro_CT) and Wolbachia sp. (Wol_CT). Additionally, columns documenting treatment group and day reclassifications are included to accurately reflect protocol adjustments performed during the study duration. This dataset serves as the primary raw source for the statistical analyses presented in the manuscript titled "Comparative study of oral treatments with milbemycin oxime and doxycycline in client-owned dogs naturally infected with Dirofilaria immitis", ensuring transparency and reproducibility in the research process.本数据集收录了一项为期12个月的对照研究的纵向监测数据，该研究针对自然感染犬恶丝虫（Dirofilaria immitis）的饲主饲养家犬，评估口服米尔贝肟（milbemycin oxime, MBO）与米尔贝肟联合多西环素（MBO+Doxy）两种疗法的应用效果。 本数据集涵盖了整个研究周期内开展的寄生虫学与分子筛查的定量检测结果。 本数据集包含的检测参数包括：经改良克诺特氏法（modified Knott’s technique）测定的微丝蚴血症水平（以每毫升血液中的微丝蚴数量量化），以及通过双重qPCR（duplex qPCR）检测得到的分子筛查结果。分子检测数据以循环阈值（Cycle Threshold, CT）值的形式呈现，该检测用于同时检测并定量犬恶丝虫及其特异性沃尔巴克氏体属（Wolbachia sp.）的DNA。 数据集包含以下列字段：用于唯一标识每只受试犬的ID编号、指定的治疗组别、以治疗后天数（days post-treatment, dpt）表示的监测时间点、微丝蚴计数（mff），以及犬恶丝虫的循环阈值（Diro_CT）与沃尔巴克氏体属的循环阈值（Wol_CT）。此外，数据集还增设了记录治疗组别与监测时间点重新分类的列字段，以准确反映研究期间对实验方案作出的调整。 本数据集为题为《自然感染犬恶丝虫的饲主饲养家犬口服米尔贝肟与多西环素疗法的对照研究》的学术论文中呈现的统计分析提供了核心原始数据来源，保障了该研究过程的透明度与可重复性。