Example Multiplexed Error-Robust Fluorescence In Situ Hybridization (MERFISH) data from the mouse colon

Multiplexed Error-Robust Fluorescence In Situ Hybridization (MERFISH) is a
massively multiplexed single-RNA-molecule imaging technique capable of
spatially resolved single-cell transcriptomic profiling of thousands of
genes in millions of cells within intact tissue slices. Initially
introduced for brain tissues, MERFISH has since been extended to other
tissues, where rapid RNA degradation during the preparation process can
pose challenges. The companion paper to these example data outlines the
application of MERFISH in one such challenging tissue, the mammalian
gastrointestinal tract. The example data contained here represent raw
MERFISH images and associated metadata necessary to illustrate the
analysis pipeline that identifies RNAs from such data.