Defective N-Glycosylation of IL6 Induces Metastasis and Tyrosine Kinase Inhibitor Resistance in Lung Cancer

The biological consequences of various IL-6 glycoforms are unknown. To uncover the function of N-glycosylation of IL-6 in lung cancer, we compare the gene expression profiles in NSCLC cells induced by conditioned medium containing IL-6 with complete N-glycosylation (NG-IL6) or defective N-glycosylation (deNG-IL6). NG-IL6- or deNG-IL6- induced gene expression in human lung adenocarcinoma cells AS2-vec (with empty vector) was measured at 3 hours after exposure to the IL-6 glycoforms 1 ng/mL. Three independent experiments were performed.