Myocardial reparative functions of exosomes from mouse mesenchymal stem cells (MSCs) are enhanced by hypoxia treatment. Mus musculus

To determine whether hypoxia augments the activity of exosomes by modulating miRNAs, we use miRNA array profile to find the differential expression of miRNAs of exosomes in different culture conditions (normoxia, hypoxia, hypoxia supplemented with GW4869 which is an inhibitor of exosomes generation). Overall design: Three samples were processed for each kind of exosome that derived from normoxic-MSCs, hypoxic-MSCs, and hypoxic-MSCs suppled with GW4869 (N-EXO, H-EXO, H+G-EXO). H-Exosome was isolated from conditioned medium of MSCs under hypoxia condtion 24h. The fragmentation mixtures were hybridized to an Agilent- Mouse microRNA array 21.0