Inherited blood cancer predisposition through altered transcription elongation [Pro-seq]

We identified CTR9 mutants as rare heritible contributing factors to several blood cancers. We later identified partial loss of CTR9 is capable of driving key hematopoietic maintenance and self-renewal gene expression through altered transcription elongation. Overall design: Examination of transcription elongation by Pro-seq on CD34+ HSPCs edited by AAVS1 and CTR9 sgRNAs