Somatic variants of MAP3K3 are sufficient to cause cerebral cavernous malformations

To further explore the underlying molecular pathways altered by MAP3K3I441M in endothelial cells, the mouse endothelial cell line bEnd.3 was first infected with lentivirus-MAP3K3I441M (LV- MAP3K3I441M-P2A-EGFP; LV-EGFP was used as the control). Total RNA from these cells was then subjected to RNA sequencing. Comparative gene expression profiling analysis of RNA seq data. We performed gene profiling analysis of RNA seq data obtained from two groups of cells.Three samples from endothelial cells with lentivirus-MAP3K3I441M infection (Mut 1–3) and five control samples with lentivirus-EGFP infection (Ctrl 1–4).