Catenulida flatworms can express genes from its microbiome or from the DNA it eats

Twenty-four hours after they were checked in a microscope to fluorescence expression and total RNA was extracted from 30 worms using TRIzol® (Invitrogen, Carlsbad, CA, USA), according to the manufacturer's instructions. For preparation of libraries, mRNA was enriched with the Dynabeads® mRNA Purification kit (Invitrogen), according to the manufacturer's protocol. Sequencing was performed in an Ion Torrent S5 sequencer (Thermo Fisher Scientific) using the Ion 540 TM Kit-OT2 and the Ion 540 TM Chip.