Data from: The pathogenesis of a 2022 North American highly pathogenic clade 2.3.4.4b H5N1 avian influenza virus in mallards (Anas platyrhynchos)

Treatment groups of ducks were exposed to different virus doses (2, 4, 6 log10 50% egg infectious doses) and by different routes (contact or intrachoanal). The experimental setting was a laboratory with animal care as approved by the institutional animal care and use committee as appropriate for the species and age of bird. Data are the virus titers shed by the oral and cloacal route for individual mallard ducks exposed to H5N1 highly pathogenic avian influenza virus by day post exposure. Samples were collected through 11 days post exposure. Virus titer equivalents were determined by quantitative real-time RT-PCR. Serological data are serum antibody titers to the challenge virus as determined by hemagglutination inhibition assay (reciprocal of the log2 dilution) determined with serum collected 10 or 11 days post exposure. Resources in this dataset:Resource Title: Virus_shed_data. File Name: Spackman_et_al_virus_shedData from H5_mallard_study_2022.csvResource Description: Virus shed data determined by quantitative real-time RT-PCRResource Title: Serology data for H5 challenged mallards. File Name: Spackman_et_al_Serology_Data from H5_mallard_study_2022.csvResource Description: Serology determined by hemagglutination inhibition assay. NS= no serum (sample not run)

本研究选取鸭类作为实验对象，将其分为不同处理组，暴露于不同的病毒剂量（2、4、6 log10 50%蛋内感染剂量）及不同途径（接触或鼻腔内）。实验环境为一符合机构动物护理和使用委员会批准标准的实验室，该标准适用于鸟类物种及年龄。数据包括个体雁鸭在暴露于H5N1高致病性禽流感病毒后，通过口腔和泄殖腔途径排出的病毒滴度，数据收集时间从暴露后第1天至第11天。病毒滴度等效值通过定量实时RT-PCR方法测定。血清学数据为挑战病毒血清抗体滴度，通过血凝抑制试验（对数稀释度的倒数）确定，血清采集时间为暴露后第10天或第11天。数据集资源包括：
资源标题：病毒排出数据。文件名：Spackman_et_al_virus_shedData_from_H5_mallard_study_2022.csv。资源描述：通过定量实时RT-PCR方法确定的病毒排出数据。
资源标题：H5挑战雁鸭的血清学数据。文件名：Spackman_et_al_Serology_Data_from_H5_mallard_study_2022.csv。资源描述：通过血凝抑制试验确定的血清学数据。NS=无血清（未进行样本测试）。