RNA-seq analysis of wildtype Aspergillus fumigatus A1160p+, ∆devR and ∆acdX in the presence and absence of Olorofim. RNA-seq analysis of wildtype Aspergillus fumigatus A1160p+, ∆devR and ∆acdX in the presence and absence of Olorofim

Deletion of transcription factors DevR and AcdX in Aspergillus fumigatus causes increased/decreased susceptibility to the antifungal Olorofim. In order to characterise the basis of differential Olorofim susceptibility, and distinguish which metabolic pathways these transcription factors regulate, RNA-seq analysis was used to compare these transcription factor knockout mutants to wildtype A. fumigatus in both the presence and absense of Olorofim.