Next Generation Sequencing facilitates the identification of differential gene expression between MNT-1 expressing control or shABCB6.

The goal of this study is to investigate the effect of ABCB6 inhibition on the transcriptome level of melanoma cell line MNT-1. We performed next-generation sequencing (NGS) on both ABCB6 depleted MNT-1 cells (shABCB6) and unknocked control cells (CTRL). Illumina mRNA-seq sample preparation kit was used to perform paired-end library sequencing with Illumina HiSeq 2500 and sequence analysis was determined using the Illumina data analysis pipeline. Based on the comparison results，we performed the GO and KEGG pathway enrichment analysis. That might suggest the key role of ABCB6 in modulating melanocyte development. Differential gene expression between MNT-1 cells expressing shcontrol or shABCB6