George E. Palade (2012) CIL:37186, Rattus, hepatocyte. CIL. Dataset

Transmission electron micrograph of thin section of rat liver with prominent Golgi and smooth ER associated vesicles. At lower right, the junction between hepatocytes harbors a bile canaliculus. The image illustrates the ethanol-stimulated accumulation of low density lipoprotein (LDL) in Golgi of the liver in rats fed ethanol (equivalent to 1 quart of single malt whisky) by gastric tube. This observation was the impetus for the strategy of purifying Golgi factions by allowing them to rise the surface of dense sucrose columns. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.

电子显微镜下观察到的鼠肝薄切片，其中高尔基体和光滑内质网相关囊泡显著。在图像右下角，肝细胞之间的连接处含有胆小管。该图像展示了在通过胃管喂养乙醇（相当于一夸脱单一麦芽威士忌）的鼠肝中，乙醇刺激的低密度脂蛋白（LDL）在高尔基体中的积累。这一观察结果推动了通过允许高尔基体组分在密集蔗糖柱表面上升的方式进行高尔基体组分纯化的策略。图像由James D. Jamieson和耶鲁大学医学院细胞生物学系提供。