Next Generation Sequencing Facilitates Quantitative Analysis of PVT1 mutant alleles have distinct growth advantage which was introduced by CRISPR in MDA-MB-231 human breast cancer cell line

PVT1 deletion alleles have growth advantage over 30 passages Overall design: Compare allele frequency between passage 0,10,20,30 in competitive growth by two independent Cas9-sgRNA RNP transfections