Next Generation Sequencing Analysis of Transcriptome Profile in CRISPR/Cas9 U90926KO IEC4.1 Cells Treated with IFN-gamma protein (IFN?)

Interferon-gamma protein (IFN?) plays a key role in intestinal epithelial cell-autonomous defense against microbial infection. The underlying mechansims of host immune regulation remains obscure and increasing evidence has shown long non coding RNAs (lncRNAs) can regulating gene expression at both the transcriptional and posttranscitpional levels. In the current study, we analyzed the transcriptome of murine intestinal epithelial cells (IEC4.1 cells) or stable CRISPR/cas9 U90926KO IEC4.1 cells following treatment with IFN? to identify potential immune gene tragets of this lncRNA. Total RNA was collected for the genome-wide analysis, for sequencing using DNBSEQ platform. Overall design: Cells were with or without IFN? treatment (2ng/ml, 4 hours). Total RNA was collected. Comparative gene expression profiling analysis of RNA-seq data for ?intestinal epithelial cells or CRISPR/Cas9 U90926KO cells with or without IFN? treatment.