Genome Wide Association Study and diversity analysis using Genotyping-by-Sequencing (GBS) of Polygonatum verticillatum Linn.. Polygonatum verticillatum

Plant secondary metabolites have a long history of being used to cure human ailments either by acting as a new structural lead in allopathy or being a part of traditional medicinal systems. Genotyping by sequencing (GBS) based on Next-generation sequencing (NGS) has widely been used for exploration of genetic diversity and breeding prospects in crop plants. In present work, GBS has been applied to a set of accessions from 66 geographical origins of Polygonatum verticillatum to identify genome-wide high quality single nucleotide polymorphisms (SNPs) for analysis of genetic diversity. This is the first report regarding generation and use of SNP markers in Polygonatum verticillatum.