Expression data from different levels of ANA in HIV+ people

Different focal adhesions, gap junction, adherens junction, and tight junction, TLR signaling pathway were seen when compared the HIV+ high ANA (anti-nuclear antibodies) group with low ANA group We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated genes during this process. HIV+ subject received the influenza vaccine, we draw the blood at the D0 and D7 of vaccination. Total RNA from purified B cells of PBMC was extracted using RNeasy Micro kit . Affymetrix Human GeneChip U133 Plus 2.0 Array was used for RNA hybridization and labeling assay. We used the gene expression to compare the gene expression of high ANA HIV+ subjects versus and low ANA subjects.