TFEB and TFE3 are required for efficient cellular response against beta-coronavirus [RNA-seq]

The transcription factors EB and E3 (TFEB and TFE3) promote lysosomal biogenesis and autophagy in response to a variety of stress conditions. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) belongs to the ß-coronaviruses family. Recent studies have shown that ß-coronaviruses use lysosomes to egress the cell. The aim of this work is to analyze if ß-coronavirus infection promotes TFEB/3 activation and to evaluate the contribution of these transcription factors to the cellular response upon viral infection. Overall design: Analysis of mRNA expression under Mouse Hepatitis Virus (MHV) infection in HeLa-mCC1a cells with siControl or siTFEB/TFE3