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Data for: An invasive ant increases deformed wing virus loads in honey bees

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Mendeley Data2024-04-13 更新2024-06-27 收录
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https://datadryad.org/stash/dataset/doi:10.5061/dryad.v15dv420f
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In the austral summer of January 2019, 18 beehives from an Argentine ant-free apiary were moved into six sites in the Northland region, New Zealand, half with known Argentine ant incursions, placing three hives in each site. Monthly collections of adult worker bees from brood frames took place from January until August, except July. Quantitative PCR (qPCR) data was generated from reverse transcribed total RNA extracted from honey bees or Argentine ants on a StepOne™ Real-Time PCR cycler or a QuantStudio 7 Real-Time PCR System, respectively. Survival data was generated from field observations. RNA-seq data originates from Illumina 1.9 Hi-seq 100 base pair (bp) paired-end sequencing of total RNA from honey bees. Reads were aligned to the honey bee reference genome (Amel_HAv3.1) in HISAT2 2.1.0 to exclude host-derived reads. Unaligned reads were de novo assembled in Trinity 2.9.1 and transcript counts generated using a pipeline integrated with the Trinity package (www.github.com/trinityrnaseq/trinityrnaseq/blob/master/util/abundance_estimates_to_matrix.pl). Further details of the experimental setup and method used can be found in the accompanying manuscript.
创建时间:
2023-06-28
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