Gene regions differentially expressed in the formaldehyde-tolerant subpopulation.

Cells were harvested in exponential growth after selection on methanol with 4 mM formaldehyde for 64 hours. Shown here are all the genes with log2 fold change >1.0 and adjusted p-value <0.001 relative to a population growing on methanol with no formaldehyde. There were three genes located within the same clusters in the genome that had no change in expression; these are also included. Expression levels in the tolerant subpopulation are shown alongside expression levels in a sensitive population experiencing formaldehyde stress (exposed to 4 mM formaldehyde for 4 hours). Log2 fold change is the mean of 3 biological replicates. Full data for all genes in the M. extorquens genome, and standard error estimates, are given in the Supporting Information, S1 Data.