Alpha-defensin binding expands human adenovirus tropism

Mammalian α-defensins are a family of abundant effector peptides of the mucosal innate immune system. Although primarily considered to be antimicrobial, α-defensins can increase rather than block infection by certain prominent bacterial and viral pathogens in cell culture and in vivo. We have shown previously that exposure of mouse and human adenoviruses (HAdVs) to α-defensins is able to overcome competitive inhibitors that block cell binding, leading us to hypothesize a defensin-mediated binding mechanism that is independent of known viral receptors. To test this hypothesis, we used genetic approaches to demonstrate that none of several primary receptors nor integrin co-receptors are needed for human α-defensin-mediated binding of HAdV to cells; however, infection remains integrin dependent. Thus, our studies have revealed a novel pathway for HAdV binding to cells that bypasses viral primary receptors. We speculate that this pathway functions in parallel with receptor-mediated entry an..., , , # Alpha-defensin binding expands human adenovirus tropism [https://doi.org/10.5061/dryad.d2547d89h](https://doi.org/10.5061/dryad.d2547d89h) ## Description of the data and file structure Files are provided for each figure as follows: Figure 1A: Scans of 96-well plates of A549 cells infected with green fluorescent protein-expressing viruses were obtained using a multi-mode plate scanner (*_platescan.tif) from 2 independent replicates (Rep##). Experimental variables include human defensin 5 (HD5) and two human adenovirus (HAdV) serotypes, C5 and D64. Samples were arranged as indicated on the plate map (.pdf). Figure 1B: Scans of 96-well plates of A549 cells infected with green fluorescent protein-expressing viruses were obtained using a multi-mode plate scanner (*_platescan.tif) from 3 independent replicates (Rep##). Samples were arranged as indicated on the plate map (.pdf). Experimental variables include wheat germ agglutinin (WGA) and human defensin 5 (HD5). From these images, tot...哺乳动物α-防御素（α-defensins）是黏膜先天免疫系统中一类丰富的效应肽家族。尽管其最初被认为具有抗菌活性，但α-防御素在细胞培养及体内实验中，反而可增强某些重要细菌与病毒病原体的感染能力。我们此前已证实，将小鼠腺病毒与人类腺病毒（human adenoviruses, HAdVs）暴露于α-防御素后，可克服阻断细胞结合的竞争性抑制剂，由此我们提出假说：存在一种由防御素介导的、不依赖已知病毒受体的病毒结合机制。为验证该假说，我们采用遗传学方法证实：人类α-防御素介导的腺病毒与细胞的结合过程，无需多种主要受体或整合素共受体参与；但病毒感染仍依赖整合素。据此，我们的研究揭示了一条绕过病毒主要受体的人类腺病毒细胞结合新途径。我们推测该途径与受体介导的入侵途径并行发挥作用…… # α-防御素结合拓展人类腺病毒宿主范围 [https://doi.org/10.5061/dryad.d2547d89h](https://doi.org/10.5061/dryad.d2547d89h) ## 数据及文件结构说明 各图片对应文件如下： ### 图1A 通过多模式平板扫描仪获取两批独立重复实验（Rep##）中，表达绿色荧光蛋白的病毒感染A549细胞的96孔板扫描图像（文件命名格式为*_platescan.tif）。实验变量包括人类防御素5（human defensin 5, HD5）以及两种人类腺病毒血清型C5与D64。样本排布详见板布局图（.pdf）。 ### 图1B 通过多模式平板扫描仪获取三批独立重复实验（Rep##）中，表达绿色荧光蛋白的病毒感染A549细胞的96孔板扫描图像（文件命名格式为*_platescan.tif）。样本排布详见板布局图（.pdf）。实验变量包括小麦凝集素（wheat germ agglutinin, WGA）与人类防御素5（HD5）。基于上述图像，总计……