Supplemental Material for Boone et al., 2020

<b>Table S1 Primers for strain construction and confirmation. </b>Primers for DJ-PCR-based fluorescent tagging were designed as previously described (Hammond <i>et al</i>. 2011b). The center DJ-PCR fragment for <i>nup120-mCherry</i> tagging was obtained from plasmid pEB1.1, which is similar to pTH1112.8 except that <i>mCherry</i> (Castro-Longoria <i>et al</i>. 2010) is present instead of <i>yfpn</i>. The center DJ-PCR fragment for <i>yfpn-cbp20</i> and <i>yfpn-sad-8</i> tagging was obtained from plasmid pNTYN-2, which is similar to pTH1123.1 except that <i>nat1</i> (Lichius and Read 2010) is present instead of <i>hph</i>.