Flow cytometry data of China

Here, the whole cultivation system was covered with sterile breathable film and acclimated to a 5-day temperature pulse cycle (25 °C for 2 days, 37 °C for 2 days and 25 °C for 1 day) in a controllable temperature incubator under a light intensity of 3000 lux with a 12 h light/12 h dark cycle. Cells reached a plateau after 5 days of growth at 25 °C (Fig. S1). At the end of the first increasing temperature cultivation, a small culture aliquot was inoculated into fresh medium to reinitiate growth for the second increasing temperature treatment. The initial cell density of each increasing temperature treatment was approximately 1 × 104 cells/mL. The control group was exposed to a constant temperature (25 °C for 5 days) for all three stages (Fig. S2). All experiments were performed in three biological replicates. Since the volume of the inoculation solution was much smaller than that of the culture system (<0.01%, v:v), the effect of inoculation on the physical and chemical properties of the culture medium was ignored. At the end of every stage, the number of living and dead cells, mean size (Size/Mean, the average of the forward scattered light intensity) and mean complexity level (Com/Mean, the average of the side scattered light intensity) of each sample were analyzed using a flow cytometer (Accuri C6 CSampler Plus, BD, USA).