Pool-seq data from laboratory selection lines of copepods collected from Kiel Canal in Germany in 2017 and 2018

The dataset contains pool-seq data from a laboratory natural selection experiment conducted on Eurytemora affinis. The E. affinis copepods used in the experiment were collected from Kiel Canal in Kiel, Germany in 2017 (approximately 1000 copepods) and on May 30, 2018 (85 gravid females and 40 juveniles). Individual adult copepods (N = 50; 25 male and 25 female) were collected for sequencing from each laboratory selection line at generations six (after one generation at 0 PSU in the treatment lines) and ten (after five generations at 0 PSU in the treatment lines). Sampled copepods from each line were pooled and their DNA was extracted using the DNeasy Blood and Tissue Extraction kit (Qiagen, Inc.). Paired-end whole-genome sequencing libraries were prepared using the Nextera DNA kit (Illumina Inc.) and sequenced on four lanes of Illumina Hi-Seq 4000 and one lane of Illumina NovaSeq 6000 at the University of Chicago Genomics Facility, generating an average of approximately 117 million paired-end (100 bp) reads per pool. These data have been deposited in NCBI under BioProject number PRJNA844002.