The mechanosensitive TRPV4 is required for crystal-induced inflammation ------ TRPV4 mediates crystal-induced inflammation

1. Macrophage-expressed TRPV4 is essential to the generation of crystal-induced joint pain and inflammation and inflammatory responses evoked by diverse crystalline structures. 2. TRPV4 is critically involved in the activation of macrophage NLRP3 inflammasome and production of the inflammatory cytokine IL-1 induced by diverse crystalline materials but not non- crystalline NLRP3 inflammasome activators. 3. A neuroimmune interaction between the TRPV1-expressing primary nociceptors and the TRPV4-expressing synovial macrophages is an important cellular mechanism behind acute gout flares.