Data for: Dissecting the genetic architecture of leaf morphology traits in mungbean (Vigna radiata (L.) Wizcek) using genome‐wide association study

Mungbean (Vigna radiata (L) Wizcek) is an important pulse crop, increasingly used as a source of protein, fiber, low fat, carbohydrates, minerals, and bioactive compounds in human diets. Mungbean is a dicot plant with trifoliate leaves. Leaves are central to various plant processes like photosynthesis, light interception, and overall canopy structure. The objectives were to study leaf morphological traits, use image analysis to extract leaf traits from images from the Iowa Mungbean Diversity (IMD) panel, develop a regression model for the prediction of leaflet area, and conduct association mapping for leaf morphological traits. We collected more than 5000 leaf images of the IMD panel consisting of 484 accessions over two years (2020 and 2021) with two replications per experiment. Leaf traits were extracted using image analysis, analyzed, and used for association mapping. Morphological diversity included leaflet type (oval or lobed), leaflet size (small, medium, large), lobed angle (shal..., Planting and experimental design
The IMD panel (Sandhu & Singh, 2021) was planted on the Iowa state Agricultural Engineering and Agronomy (AEA) Farm (Latitude: 42.02°, Longitude: -93.78°) in Boone, Iowa (IA). In 2020, planting was done on the 5th of June at the Burkey and Bruner farms, while in 2021, planting was done on the 3rd of June at the AEA and Bruner farms. The farms can be viewed here using the ISU Lands app.  Each accession was planted in 7ft single-row plots consisting of 50 plants. A 2” and 30” spacing was used between plants in a plot and between plots, respectively. A randomized complete block design (RCBD) was used with two replicates at each location. Standard agronomic practices were used in the management of the crop.
Leaf collection, image capture, and trait extraction
Leaves were collected from one replication per location for the two years giving us four data points. Leaf collections were done during the vegetative growth and took between three to five days at e..., Any software that can view images
You extract the data using the open-source software at https://bitbucket.org/baskargroup/leaf_annotator/src/master/
The code can be accessed at https://github.com/yalek/mungbean_leaf_gwas