Experimental PCR Data on Soil DNA Extracts

Bacillus species and B. anthracis presence/absence data were determined in 4,770 soil samples collected across the contiguous United States, in cooperation with the U.S. Environmental Protection Agency (EPA). Polymerase Chain Reaction (PCR) data for Bacillus species and B. anthracis rpoB gene PCR amplicon detection were reported as non-detect (n), low (l), medium (m), and high (h). Results for both pag and lef genes of the pX01 plasmid were reported by the University of South Florida's Center for Biological Defense. This data was recorded as negative or positive for each of the genes and included the following combinations: neg/neg, pos/neg, neg/pos, and pos/pos. Data for the pX02 plasmid were recorded as negative (neg) or positive (pos).本数据集包含美国本土采集的4770份土壤样本的检测数据，其中芽孢杆菌属（Bacillus species）与炭疽芽孢杆菌（B. anthracis）的存在/缺失情况已明确，相关检测工作与美国环境保护署（U.S. Environmental Protection Agency, EPA）合作开展。针对芽孢杆菌属及炭疽芽孢杆菌rpoB基因的聚合酶链反应（Polymerase Chain Reaction, PCR）扩增子检测结果分为四类：未检出（n）、低浓度（l）、中浓度（m）与高浓度（h）。美国南佛罗里达大学生物防御中心报告了pX01质粒上pag与lef双基因的检测结果：每类基因的检测结果以阴性或阳性标记，组合类型包括neg/neg、pos/neg、neg/pos及pos/pos。pX02质粒的检测结果则以阴性（neg）或阳性（pos）记录。