Raw spectral data - Membrane Profiling by Free Flow Electrophoresis and SWATH-MS to Characterize Subcellular Compartment Proteomes in Mesembryanthemum crystallinum

The study of subcellular membrane structure and function facilitates investigations into how biological processes are divided within the cell. However, work in this area has been hampered by the limited techniques available to fractionate the different membranes. Free-Flow Electrophoresis (FFE) allows for the fractionation of membranes based on their different surface charges, a property made up primarily of their varied lipid and protein compositions. In this study, high-resolution plant membrane fractionation by FFE, combined with mass spectrometry-based proteomics, allowed the simultaneous profiling of multiple cellular membranes from the leaf tissue of the plant Mesembryanthemum crystallinum. Comparisons of the fractionated membranes protein profile to that of known markers for specific cellular compartments sheds light on the functions of proteins as well as provides new evidence for multiple subcellular localization of several proteins, including those involved in lipid metabolism. The raw spectral data from the experiments in this study are available through this dataset release.

对亚细胞膜结构与功能的研究，可为解析细胞内生物学过程的空间分隔机制提供重要支撑。然而，该领域的研究长期受制于可用的不同膜组分分级分离技术的匮乏。自由流电泳（Free-Flow Electrophoresis, FFE）可依据膜的表面电荷差异实现膜组分的分级分离，而表面电荷主要由膜独特的脂质与蛋白质组成所决定。本研究中，研究人员结合基于质谱的蛋白质组学技术，通过高分辨率自由流电泳对冰叶日中花（Mesembryanthemum crystallinum）叶片组织中的多种细胞膜组分进行分级分离，并同步完成了各组分的蛋白质谱分析。将分离得到的膜蛋白谱与特定细胞区室的已知标志物蛋白谱进行比对，不仅可阐明蛋白质的功能，还为多种蛋白质的多亚细胞定位提供了新证据，其中包括参与脂质代谢的相关蛋白。本研究实验所产生的原始质谱谱图数据，已随本数据集一同公开。