Normalized oxygen from incubations conducted with methylcyclopentane using samples collected from the Gulf of Mexico during June 2015 on R/V Atlantis cruise AT29-02

Hydrocarbon incubations were conducted with water collected from 1000 meters depth in the Gulf of Mexico across four stations during cruise AT29-02 on the R/V Atlantis. Sample collection occurred June 16-17, 2015. Each incubation was injected with 10 microliters of methylcyclohexane (MCH) or methylcyclopentane (MCP). Each incubation was monitored for oxygen content using remote optical oxygen sensors. After we observed a respiration signal, we sacrificially harvested each bottle for cell counts, nutrients, and DNA. Incubation time varied from 10-31 days. These data were used to assess who consumes these compounds in the deep Gulf of Mexico, over what time frame, and what metabolic pathways bacteria use to consume them. This dataset includes the normalized oxygen measurements from the MCP incubations.本研究依托“亚特兰蒂斯号”（R/V Atlantis）科考船执行的AT29-02航次，采集了墨西哥湾1000米水深的4个站位的海水样品，用于开展烃类培养实验。样品采集工作于2015年6月16日至17日完成。每组培养体系均被注入10微升的甲基环己烷（methylcyclohexane, MCH）或甲基环戊烷（methylcyclopentane, MCP）。采用远程光学氧传感器对每组培养体系的溶解氧含量进行监测。当观测到微生物呼吸信号后，我们对每一组培养瓶进行牺牲式取样，用于细胞计数、营养盐检测以及DNA分析。培养时长介于10至31天之间。本数据集相关数据被用于解析墨西哥湾深海环境中哪些微生物可代谢此类化合物、代谢发生的时间范围，以及细菌降解此类化合物所采用的代谢通路。本数据集包含甲基环戊烷（MCP）培养体系的标准化氧含量测定数据。