Estimated tree using data from Johnson et al., (2009)

Our estimated tree generated using data from Johnson et al., (2009). The tree is based on the nuclear gene (EF-1α) and mitochondrial genes (12S and COI) for 38 species in the genus Columbicola and one outgroup, Oxyliperurus chiniri. The accession numbers were taken from Johnson et al., (2007). The tree was estimated using BEAST v2.1.3 (Bouckaert et al., 2014), allowing the topology to vary. We used a GTR + Γ model of nucleotide evolution with separate partitions for each gene and codon position, a relaxed log-normal clock, and a birth-death prior. Further information and full references can be found in the supplementary material of our paper.