Additional file 1 of A new strain of Neowestiellopsis (Hapalosiphonaceae): first observation of toxic soil cyanobacteria from agricultural fields in Iran

Additional file 1: Supplementary Fig. S1. Life cycle of the new Neowestiellopsis ca. persica isolated from Iran. a) Hormogonium, b) filament from hormogonium, c) Akinets, d) Akinets germination, e) multiseriate filament from akinets, f) Monocyte formation from the ends of filaments, g) Monocyte with heterocyte, h) little heteropolar filaments from Monocites, i) heteropolar filaments, j) typical Neowestiellopsis filament. Supplementary Fig. S2. Phylogenetic position of studied strain (Highlighted in red) and related cyanobacteria based on mcy G gene with Microcystis aeruginosa (AB110133) as out-group. Numbers near nodes indicate standard bootstrap support (%)/ultrafast bootstrap support (%) for ML analyses. Supplementary Fig. S3. Phylogenetic position of studied strain (Highlighted in red) and related cyanobacteria based on mcy D gene with PCC_7806SL (CP020771) as out-group. Numbers near nodes indicate standard bootstrap support (%)/ultrafast bootstrap support (%) for ML analyses. Supplementary Fig. S4. Phylogenetic position of studied strain (Highlighted in red) and related cyanobacteria based on nos gene. Numbers near nodes indicate standard bootstrap support (%)/ultrafast bootstrap support (%) for ML analyses. Table S1. Target genes and oligonucleotide primers used in this study. Table S2. Accession numbers of Sequence data deposited in the DNA Data Bank of Japan. Table S3. Comparison of the nucleotides length of the ITS regions of Neowestiellopsis persica with reference strains. Table S4. Comparison of secondary structure of 16S-23S rRNA (D1-D1, helix and Box-B helix) between the Neowestiellopsis persica and related taxa. Table S5. Comparison of secondary structure of 16S-23S rRNA (D2, helix and V3) between the Neowestiellopsis persica and related taxa.