Colocalized synaptophysin-GFP puncta.

Parameters of the model used to fit the data on colocalized synaptophysin-GFP puncta. The model starts with 100 occupied sites, of which N2 are stable for the entire experiment, and N1 unoccupied sites. In each 10 min time interval, the probability of a site going from unoccupied to occupied is P2, the probability of going from occupied to unoccupied is (1 - P1), and the probability of going from present to absent (starting at 10 min) is (1 - P3). An increase in N1 (for example, comparing glutamate to control) corresponds to a rapid increase in sites at 10 min, an increase in P3 corresponds to a gradual increase (or a smaller decrease) from 10 min to 3 hrs, and an increase in N2 corresponds to an increase in stable sites.