High-throughput screening of differentially expressed lncRNAs in peripheral blood plasma between coronary artery disease patients and controls

This study aims to discover unknown LncRNAs associated with Coronary Artery Disease(CAD)in peripheral blood. Three pairs of clinical CAD group and control blood plasma samples were texted in this experiment by high-throughput sequencing methods. Cuffdiff software was used to get the expression profiles of LncRNAs, differentially expressed LncRNAs were identified based on fold change and p-value. Differently expressed genes in peripheral plasma and blood monocytes were verified by Q-PCR in large samples of clinical CAD group and controls. We find three LncRNAs uc003pxg.1, ENST00000565257, ENST00000568324 were up-regulated obviously in peripheral blood mononuclear cells of CAD patients and these outcomes were consistent with the sequencing results. The ideal LncRNAs were detected preliminarily. This founding lays the foundation for further research,and provides theoretical basis of LncRNA using for CAD early clinical screening. Overall design: Differentially expressed lncRNAs of 6 clinal samples were examinated including 3 clinical CAD and 3 control blood plasma samples.